diff --git a/Gemfile b/Gemfile
index ce1bed6cc88..2c7651ed3f0 100644
--- a/Gemfile
+++ b/Gemfile
@@ -188,7 +188,7 @@ group :unicorn do
 end
 
 group :puma do
-  gem 'puma', '~> 5.1.1', require: false
+  gem 'puma', '~> 5.3.1', require: false
   gem 'puma_worker_killer', '~> 0.3.1', require: false
 end
 
diff --git a/Gemfile.lock b/Gemfile.lock
index 5be9b9b8a58..56310ec6b89 100644
--- a/Gemfile.lock
+++ b/Gemfile.lock
@@ -935,7 +935,7 @@ GEM
       tty-markdown
       tty-prompt
     public_suffix (4.0.6)
-    puma (5.1.1)
+    puma (5.3.2)
       nio4r (~> 2.0)
     puma_worker_killer (0.3.1)
       get_process_mem (~> 0.2)
@@ -1577,7 +1577,7 @@ DEPENDENCIES
   pry-byebug
   pry-rails (~> 0.3.9)
   pry-shell (~> 0.4.0)
-  puma (~> 5.1.1)
+  puma (~> 5.3.1)
   puma_worker_killer (~> 0.3.1)
   rack (~> 2.2.3)
   rack-attack (~> 6.3.0)
diff --git a/config/feature_flags/development/webauthn.yml b/config/feature_flags/development/webauthn.yml
index 5339074e84a..0dc9e2b7bfd 100644
--- a/config/feature_flags/development/webauthn.yml
+++ b/config/feature_flags/development/webauthn.yml
@@ -1,7 +1,7 @@
 ---
 name: webauthn
 introduced_by_url: https://gitlab.com/gitlab-org/gitlab/-/merge_requests/26692
-rollout_issue_url: 
+rollout_issue_url: https://gitlab.com/gitlab-org/gitlab/-/issues/232671
 milestone: '13.4'
 type: development
 group: group::access
diff --git a/config/initializers/puma_client_tempfile_patch.rb b/config/initializers/puma_client_tempfile_patch.rb
deleted file mode 100644
index 972eeaf0c83..00000000000
--- a/config/initializers/puma_client_tempfile_patch.rb
+++ /dev/null
@@ -1,101 +0,0 @@
-# frozen_string_literal: true
-
-if Gitlab::Runtime.puma?
-  # This patch represents https://github.com/puma/puma/pull/2613. If
-  # this PR is accepted in the next Puma release, we can remove this
-  # entire file.
-  #
-  # The patch itself is quite large because the tempfile creation in
-  # Puma is inside these fairly long methods. The actual changes are
-  # just two lines, commented with 'GitLab' to make them easier to find.
-  raise "Remove this monkey patch: #{__FILE__}" unless Puma::Const::VERSION == '5.1.1'
-
-  module Puma
-    class Client
-      private
-
-      def setup_body
-        @body_read_start = Process.clock_gettime(Process::CLOCK_MONOTONIC, :millisecond)
-
-        if @env[HTTP_EXPECT] == CONTINUE
-          # TODO allow a hook here to check the headers before
-          # going forward
-          @io << HTTP_11_100
-          @io.flush
-        end
-
-        @read_header = false
-
-        body = @parser.body
-
-        te = @env[TRANSFER_ENCODING2]
-
-        if te
-          if te.include?(",")
-            te.split(",").each do |part|
-              if CHUNKED.casecmp(part.strip) == 0 # rubocop:disable Metrics/BlockNesting
-                return setup_chunked_body(body)
-              end
-            end
-          elsif CHUNKED.casecmp(te) == 0
-            return setup_chunked_body(body)
-          end
-        end
-
-        @chunked_body = false
-
-        cl = @env[CONTENT_LENGTH]
-
-        unless cl
-          @buffer = body.empty? ? nil : body
-          @body = EmptyBody
-          set_ready
-          return true
-        end
-
-        remain = cl.to_i - body.bytesize
-
-        if remain <= 0
-          @body = StringIO.new(body)
-          @buffer = nil
-          set_ready
-          return true
-        end
-
-        if remain > MAX_BODY
-          @body = Tempfile.new(Const::PUMA_TMP_BASE)
-          @body.binmode
-          @body.unlink # GitLab: this is the changed part
-          @tempfile = @body
-        else
-          # The body[0,0] trick is to get an empty string in the same
-          # encoding as body.
-          @body = StringIO.new body[0,0] # rubocop:disable Layout/SpaceAfterComma
-        end
-
-        @body.write body
-
-        @body_remain = remain
-
-        return false # rubocop:disable Style/RedundantReturn
-      end
-
-      def setup_chunked_body(body)
-        @chunked_body = true
-        @partial_part_left = 0
-        @prev_chunk = ""
-
-        @body = Tempfile.new(Const::PUMA_TMP_BASE)
-        @body.binmode
-        @body.unlink # GitLab: this is the changed part
-        @tempfile = @body
-        @chunked_content_length = 0
-
-        if decode_chunk(body)
-          @env[CONTENT_LENGTH] = @chunked_content_length
-          return true # rubocop:disable Style/RedundantReturn
-        end
-      end
-    end
-  end
-end