diff --git a/app/assets/stylesheets/pages/notes.scss b/app/assets/stylesheets/pages/notes.scss
index adab2253365..7e7eff1346a 100644
--- a/app/assets/stylesheets/pages/notes.scss
+++ b/app/assets/stylesheets/pages/notes.scss
@@ -494,11 +494,6 @@ $note-form-margin-left: 72px;
   .discussion-notes {
     margin-left: 0;
     border-left: 0;
-
-    .notes {
-      position: relative;
-      @include vertical-line(52px);
-    }
   }
 
   .note-wrapper {
diff --git a/changelogs/unreleased/57768-remove-vertical-line.yml b/changelogs/unreleased/57768-remove-vertical-line.yml
new file mode 100644
index 00000000000..b73b0fa229e
--- /dev/null
+++ b/changelogs/unreleased/57768-remove-vertical-line.yml
@@ -0,0 +1,5 @@
+---
+title: Remove vertical connecting line placeholder from diff discussion notes
+merge_request: 25292
+author:
+type: fixed